SAB4200109

Anti-PIWIL1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-HIWI, Anti-MIWI, Anti-PIWI-like 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~95 kDa
• species reactivity: human, mouse
• packaging: antibody small pack of 25 μL
• concentration: ~1.0 mg/mL
• technique(s): immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded mouse testis and biotin / ExtrAvidinTM-Peroxidase staining system.; western blot: 1-2 μg/mL using lysates of HEK-293Tcells over expressing human PIWIL1.
• UniProt accession no.: Q96J94
• shipped in: dry ice
• Storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PIWIL1(9271)

Description

General description

The PIWI-like protein 1 (PIWIL1) belongs to the Piwi subfamily.gene is mapped to human chromosome 12q24.33. It is expressed in the cytosol of spermatocytes and spermatids. PIWI-like protein 1 (PIWIL1) is also an Argonaute protein family member.

Specificity

Anti-PIWIL1 recognizes human and mouse PIWIL1.

Application

Anti-PIWIL1 antibody produced in rabbit has been used in western blotting and immunohistochemistry.

Biochem/physiol Actions

The PIWI-like protein 1 (PIWIL1) mediates spermatogenesis. Its aberrant expression favors tumor progression inadenocarcinoma. PIWI-like protein 1 (PIWIL1) plays a key role in embryogenesis, differentiation, stem cell proliferation, growth, and development. High levels of PIWIL1 expression is implicated in the colorectal cancer invasion.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable