SAB4200104

Anti-Gigaxonin (N-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

• Synonym(s): Anti-GAN, Anti-GAN1, Anti-Giant axonal neuropathy 1, Anti-KLHL16 (kelch-like protein 16)
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~70 kDa
• species reactivity: mouse, human, rat
• concentration: ~1.5 mg/mL
• technique(s): immunoprecipitation (IP): 15-30 μg using rat brain extracts (S1 fraction); indirect immunofluorescence: 10-20 μg/mL using HeLa cells expressing human gigaxonin; western blot: 1-2 μg/mL using mouse brain extracts (S1 fraction), and 1.5-3 μg/mL using SH-SY5Y cell lysates; western blot: 1.5-3 μg/mL using SH-SY5Y cell lysates
• UniProt accession no.: Q9H2C0
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... GAN(8139) ; mouse ... Gan(209239) ; rat ... Gan(307893)

Description

General description

Gigaxonin (GAN) is encoded by the gene mapped to human chromosome 16q23.2. The encoded protein is characterized with an N-terminal BTB (for broad-complex, ramtrack and bric a brac) domain followed by a six kelch repeats, which are predicted to adopt a β-propeller shape.8

Application

Anti-Gigaxonin (N-terminal) antibody produced in rabbit has been used in immunofluorescence and immunoblotting assay.[1]

Biochem/physiol Actions

Gigaxonin (GAN) acts as a key regulator of intermediate filaments (IFs) and the microtubule-associated protein 1B (MAP1B) light chain degradation, which is required for neuronal survival. GAN contributes to neurofilament architecture. Additionally, it also influences the ubiquitination and degradation of some proteins. Mutation in the gene is associated with the development of giant axonal neuropathy.[1]

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable