SAB4200099

Anti-Gapex-5 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-GAPVD1, Anti-GTPase-activating protein and VPS9 domains 1, Anti-RAB5 exchange factor, Anti-RAP6 (rab5-activating protein 6)
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~180 kDa
• packaging: antibody small pack of 25 μL
• concentration: ~1.0 mg/mL
• technique(s): western blot: 1-2 μg/mL using whole extracts of human HeLa cells
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... GAPVD1(26130) ; mouse ... Gapvd1(66691) ; rat ... Gapvd1(311880)

Description

General description

The GTPase activating protein and VPS9 domains 1 (Gapex-5) protein, originally known as receptor-mediated endocytosis protein 6 (RME-6)/Rab5-activating protein 6 (RAP6), is a guanine nucleotide exchange factor (GEF) for the Rab5 subfamily of guanine triphosphatase (GTPases). Gapex-5 is an evolutionarily conserved protein with Rab5 GEF domain, the N-terminal Ras GTP hydrolysis-activating protein (GAP) domain and a C-terminal Vps9 domain (a Rab5 guanine diphosphate (GDP)/GTP exchange factor). Gapex-5 is localized on the plasma membrane and on endosomes. This gene is mapped to human chromosome 9q33.3.

Specificity

Anti-Gapex-5 (N-terminal) recognizes human Gapex-5.

Application

Anti-Gapex-5 (N-terminal) antibody produced in rabbit is suitable for immunoblotting.

Biochem/physiol Actions

The GTPase activating protein and VPS9 domains 1 (Gapex-5) protein has guanine nucleotide exchange factor (GEF) activity specific for Rab5 and guanine triphosphate (GTP) hydrolysis-activating protein (GAP) activity specific for Ras. It is involved in multiple processes including receptor-mediated endocytosis, insulin receptor internalization, glucose transporter 4 (Glut4) trafficking in adipocytes, epidermal growth factor receptor (EGFR) trafficking and degradation and phagosome maturation.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable