Monoclonal Anti-OSTF1 (mouse IgG2b isotype) is derived from the hybridoma OSTF21 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide.The osteoclast-stimulating factor (OSF or OSTF1) contains a short proline-rich N-terminal region, an SH3 domain, three ankyrin repeats and an aspartate rich C-terminal region. It is a novel 28 kDa intracellular protein predominantly expressed in osteoclast (OCL). The OSTF1 gene is mapped to human chromosome 9q21.13.
Specificity
Monoclonal Anti-OSTF1 recognizes human, monkey, bovine, rat, and mouse OSTF1.
Application
Anti-OSTF1 antibody, Mouse monoclonal is suitable for immunoblotting and immunofluorescence.
Biochem/physiol Actions
Osteoclast-stimulating factor (OSF or OSTF1) is potentially involved in protein-protein interactions. It supports bone resorption by cellular signal transduction cascade. OSTF1 induces osteoclast (OCL) bone formation by its SH3 domain interacting with spinal muscular atrophy disease determining gene product, survival motor neuron (SMN). The OSF SH3-SMN interaction might be significant in congenital bone fractures associated with type I spinal muscular atrophy disease. Repressed, OSF-SH3 domain interaction with its protein partners might cause reduced bone resorption by OCL. This prevents the bone loss that is associated with many bone diseases, such as osteoporosis, periodontal disease, Paget′s disease, estrogen deficiency, inflammatory bone loss, hyperparathyroidism and bone malignancy.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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