SAB4200090

Anti-Raver1 (C-terminal) antibody produced in rabbit

IgA fraction of antiserum

• Synonym(s): Anti-Ribonucleoprotein PTB-binding 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgA fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~77 kDa
• technique(s): immunoprecipitation (IP): 5-10 μL using lysates of HEK-293Tcells overexpressing human Raver1; indirect immunofluorescence: 1:500-1:1000 using paraformaldehyde fixed HEK-293T cells overexpressing human Raver1; western blot: 1:500-1:1000 using lysates of HEK-293T cells overexpressing human Raver1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RAVER1(125950) ; mouse ... Raver1(71766) ; rat ... Raver1(298705)

Description

General description

Raver1 is encoded by the gene mapped to human chromosome 19p13.2.

Raver1, also known as ribonucleoprotein phosphotyrosine-binding (PTB)-1, is a widely expressed multidomain protein. Raver1 is composed of three RNA recognition motifs (RRM) and both nuclear localization and nuclear export signals. It thus, shuttles between the nucleus and the cytoplasm. Raver1 also colocalizes with PTB/hnRNPI, a protein involved in RNA splicing of microfilament proteins.

Specificity

Anti-Raver1 (C-terminal) recognizes human Raver1.

Application

Anti-Raver1 (C-terminal) antibody produced in rabbit is suitable for:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions

Variation in the raver1 gene expression may be associated with the development of depressive disorder. In skeletal muscle, migration of of Raver1 from the nucleus to the cytoplasm facilitates differentiation of specific microfilament attachment sites.

Vinculin recruits raver1 and its mRNAs cargo to focal adhesions, promoting localization of the synthesis of adhesion complexes by the translational machinery.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable