SAB4200088

Anti-DGCR8 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-DGCRK8, Anti-DiGeorge syndrom critical region 8
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~100 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 2.5-5 μg using lysates of HEK-293T cells over expressing human DGCR8; indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HEK-293T cells over expressing human DGCR8; western blot: 1-2 μg/mL using lysates of HEK-293T cells over expressing human DGCR8
• UniProt accession no.: Q8WYQ5
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... DGCR8(54487) ; mouse ... Dgcr8(94223)

Description

General description

DGCR8 (DGCR8 microprocessor complex subunit) is a double-stranded RNA-binding protein mapped to the chromosome 22q11.2. It is composed of a WW domain and two double-stranded RNA-binding domains (dsRBDs).[1]

Application

Anti-DGCR8 (C-terminal) antibody produced in rabbit has been used in western blot assay to study the subcellular localization of KSRP (KH-type splicing regulatory protein) in RACK1 (receptor for activated protein kinase C)-knockdown cells. It has been used in Western blotting to study the phosphorylation status of TRBP (TAR RNA binding protein) in a study of glucose tolerance through inhibition of microRNA maturation in miRNA103 transgenic mice.[2] Anti-DGCR8 (C-terminal) antibody produced in rabbit is also suitable for immunoprecipitation at a concentration of 2.5-5μg using lysates of HEK-293T cells over expressing human DGCR8, indirect immunofluorescence at the concentration of 2-5μg/mL using paraformaldehyde fixed HEK-293T cells over expressing human DGCR8 and western blot at a dilution of 1-2μg/mL using lysates of HEK-293T cells over expressing human DGCR8.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

DGCR8 (DGCR8 microprocessor complex subunit) participates in the biogenesis of microRNA (miRNA, miR) as a major component of the microprocessor complex. DGCR8 provides guidance to the RNase III enzyme, Drosha during rRNA processing. It forms the microprocessor complex by binding to the RNase III enzyme Drosha, which further converts long primary miRNAs (pri-miRNAs) into short hairpins called precursor miRNAs (pre-miRNAs). The processed hairpins finally are transported to the cytoplasm for further processing by Dicer into mature miRNAs. DGCR8 is also required for global gene regulation and silencing of embryonic stem cell self-renewal. Deletion of DGCR8 chromosomal location has been reported in the DiGeorge and velocardiofacial syndrome.[1]

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable