SAB4200076

Anti-Nedd9 (C-terminal) antibody produced in rabbit

affinity isolated antibody

• Synonym(s): Anti-Cas-L, Anti-HEF1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~115 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): indirect immunofluorescence: 1-2 μg/mL using human HS68 cells; western blot: 1.5-3.0 μg/mL using extracts of HEK-293T cells overexpressing human Nedd9
• UniProt accession no.: Q14511
• shipped in: dry ice
• Storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NEDD9(4739) ; mouse ... Nedd9(18003) ; rat ... Nedd9(291044)

Description

General description

Neural precursor cell expressed, developmentally down-regulated 9 (Nedd9), also known as Cas-L and human enhancer of filamentation 1 (HEF1) is an adhesion adapter protein, localized to sites of focal adhesions. It belongs to a larger family of Cas docking adapter proteins including p130Cas and Efs and is expressed preferentially in lymphocytes and epithelial cells. The gene NEDD9 is mapped to human chromosome 6p24.2.

Specificity

Anti-Nedd9 (C-terminal) specifically recognizes human Nedd9.

Application

Anti-Nedd9 (C-terminal) antibody produced in rabbit is suitable for immunoblotting and immunofluorescence.

Biochem/physiol Actions

Neural precursor cell expressed, developmentally down-regulated 9 (Nedd9) protein participates in integrin and growth factor signaling pathways and regulates growth, motility and apoptosis. It undergoes extensive phosphorylation, a process dependent both on cell adhesion and on the integrity of actin cytoskeleton. In response to integrin receptor binding to the extracellular matrix (ECM), Nedd9 binds to focal adhesion kinase (FAK) and is phosphorylated by FAK and Src kinases. In addition, Nedd9 is regulated at multiple levels in a complex and cell cycle-dependent manner. It is thought to play an important role in early neuronal differentiation and brain development. Nedd9 is also a key player in metastatic cancer. Upregulation of Nedd9 expression is observed in various solid tumors and is greatly implicated in the pathogenesis of BCR-Abl-dependent tumors. Overexpression of Nedd9 has been shown to be required for invasion by glioblastoma and to be strongly linked to the promotion of melanoma metastasis.

Physical form

Solution in 0.01 M posphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable