SAB4200066

Anti-EIF2C1/Ago1 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-Argonaute-1, Anti-Eukaryotic translation initiation factor 2C, subunit 1, Anti-GERP95, Anti-Golgi Endoplasmic Reticulum protein 95 kDa
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~100 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells over expressing hEIF2C1/Ago1; indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HEK-293T cells over expressing hEIF2C1/Ago1; western blot: 0.5-1 μg/mL using lysates of HEK-293T cells over expressing hEIF2C1/Ago1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... EIF2C1(26523) ; mouse ... Eif2c1(236511) ; rat ... Eif2c1(313594)

Description

General description

In humans, the argonaute (Ago) subfamily consists of four isoforms hsAgo1−4. Ago proteins (~100 kDa molecular weight) contain a piwi-argonaute-zwille (PAZ) and PIWI domains. These proteins are evolutionarily conserved between species. EIF2C1/Ago1 is predominantly expressed in embryonic kidney and lung.

The eukaryotic initiation factor (EIF2C1), also known as argonaute 1, RNA-induced silencing complex (RISC) catalytic component, is encoded by the gene with 19 exons and 18 introns spanning ~ 50 kb on genomic DNA, mapped to human chromosome 1p34.3. The gene is widely expressed at low and medium levels. The encoded protein is a member of argonaute family of proteins.

Application

Anti-EIF2C1/Ago1 (N-terminal) antibody produced in rabbit has also been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Anti-EIF2C1/Ago1 (N-terminal) antibody produced in rabbit has been used in chromatin immunoprecipitation (ChIP) and RNA-native ChIP (NChIP) experiments.

Biochem/physiol Actions

EIF2C1 is implicated in both transcriptional and post-transcriptional gene silencing. EIF2C1 levels are also increased in tumors that lack the Wilm′s tumor suppressor gene, WT1.

Eukaryotic initiation factor (EIF2C1)/ argonaute 1 plays a vital role in activation of transcriptional enhancers and also regulates alternative splicing in human cells. The encoded protein interacts with hypoxia-responsive microRNAs (HRMs) and stimulate angiogenic pathway.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable