SAB4200064

Anti-Snurportin 1 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-RNA U transporter 1, Anti-RNUT1, Anti-SNUPN
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~41 kDa
• packaging: antibody small pack of 25 μL
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 2.5-5 μg using lysates of HEK-293T cells over expressing human Snurportin 1; western blot: 1-2 μg/mL using lysates of HEK-293T cells over expressing human Snurportin 1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SNUPN(10073)

Description

General description

Snurportin 1 (also known as RNA U transporter 1, SPN1 and SNUPN) comprises three known functional domains: an N-terminal importin-β binding domain (IBB) motif and trimethylguanosine (TMG) cap-binding domain. SNUPN is mapped to human chromosome 15q24.2.

Specificity

Anti-Snurportin 1 (N-terminal) recognizes human snurportin 1.

Application

Anti-Snurportin 1 (N-terminal) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Snurportin 1 (also known as RNA U transporter 1, SPN1 and SNUPN), mediates nuclear import of 7-methylguanosine (m⁷G) and trimethylguanosine (TMG)-capped uridine-rich small nuclear RNPs (U snRNPs), an important step in the maturation of the spliceosome. The N-terminal importin-β binding domain (IBB) motif, which binds importin β that mediates the interaction with the nuclear pore complex (NPC). The TMG cap-binding domain interacts specifically with m³G-cap but not with m⁷G-cap structures, and an ill-defined region responsible for binding to exportin 1. The active nuclear import, entry of spliceosomal U snRNPs into the nucleus by the importin b/Snurportin1/U snRNP complex does not require RanGTP. Snurportin 1 accumulates in Cajal bodies after import of U2 but not U1 snRNPs.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable