immunoprecipitation (IP): 2.5-5 μg using lysates of HEK-293T cells over expressing human Snurportin 1 western blot: 1-2 μg/mL using lysates of HEK-293T cells over expressing human Snurportin 1
Snurportin 1 contains an N-terminal importin β binding (IBB) motif, 2,2,7-trimethylguanosine (TMG) cap-binding domain, an ill-defined region and a C‐terminal m3Gcap-binding region. The SNUPN gene is mapped on the human chromosome at 15q24.2.
Specificity
Anti-Snurportin 1 recognizes human snurportin 1.
Application
Anti-Snurportin 1 (C-terminal) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.
Biochem/physiol Actions
Snurportin 1 mediates the nuclear import of 5′‐2,2,7‐terminal trimethylguanosine (m3G)-capped uridine-rich small nuclear RNPs (U snRNPs), an important step in the maturation of the spliceosome. This protein binds with importin β that mediates the interaction with the nuclear pore complex (NPC). It also interacts with m3G-cap but not with 5′‐terminal 7‐monomethylguanosine (m7G)-cap structures and chromosomal maintenance 1 (Crm1)/exportin 1. Snurportin 1 was found to accumulate in Cajal bodies after the import of U2 but not U1 snRNPs.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
The nuclear import of the spliceosomal snRNPs U1, U2, U4 and U5, is dependent on the presence of a complex nuclear localization signal (NLS). The latter is composed of the 5'-2,2,7-terminal trimethylguanosine (m3G) cap structure of the U snRNA and
Molecular biology of the cell, 16(10), 4660-4671 (2005-07-21)
The initial steps of spliceosomal small nuclear ribonucleoprotein (snRNP) maturation take place in the cytoplasm. After formation of an Sm-core and a trimethylguanosine (TMG) cap, the RNPs are transported into the nucleus via the import adaptor snurportin1 (SPN) and the
We report the first patient with an interstitial deletion of chromosome 15q24.1-q24.3 associated with common variable immunodeficiency (CVID). The 18-year old female patient's clinical and immunological phenotype was compared with 8 additional previously published patients with chr15q24 deletions. A CGH
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