SAB4200052
Anti-JUB (N-terminal) antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-Ajuba
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~57 kDa
species reactivity
mouse, human
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~1.5 mg/mL
technique(s)
indirect immunofluorescence: 7.5-15 μg/mL using P19 cells
western blot: 1-2 μg/mL using HeLa cell lysates
shipped in
dry ice
Storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... JUB(84962)
General description
JUB also is known as Ajuba is a member of the zyxin family of cytosolic Lin11, Isl-1, Mec-3 (LIM)proteins. This protein is expressed in skin, kidney, liver, lung, and genitourinary organs. The JUB gene is associated with the human chromosome at 14q11.2.
Specificity
Anti-JUB (N-terminal) specifically recognizes human and mouse JUB.
Application
Anti-JUB (N-terminal) antibody produced in rabbit may be used in immunoblotting and immunofluorescence.
Biochem/physiol Actions
JUB protein mediates various cellular events. It plays a role in cadherin-mediated cell-cell adhesion and cell migration. This protein is recruited to cadherin complexes through interaction with α-catenin. JUB protein also influences the localization of Crk-associated substrate (p130Cas) at focal adhesions and Rac family small guanosine triphosphatase (GTPase 1) (Rac1) activity during cell migration. This protein shuttles between the cytoplasm and the nucleus to affect embryonal cell proliferation. JUB protein interacts with and activates Aurora-A and is required for mitotic commitment in human cells.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Stephen J Pratt et al.
The Journal of cell biology, 168(5), 813-824 (2005-02-25)
Cell migration requires extension of lamellipodia that are stabilized by formation of adhesive complexes at the leading edge. Both processes are regulated by signaling proteins recruited to nascent adhesive sites that lead to activation of Rho GTPases. The Ajuba/Zyxin family
Helene Marie et al.
The Journal of biological chemistry, 278(2), 1220-1228 (2002-11-06)
Cell-cell adhesive events affect cell growth and fate decisions and provide spatial clues for cell polarity within tissues. The complete molecular determinants required for adhesive junction formation and their function are not completely understood. LIM domain-containing proteins have been shown
Marina Kisseleva et al.
Molecular and cellular biology, 25(10), 3956-3966 (2005-05-05)
The phosphoinositide phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] regulates the activity of many actin-binding proteins and as such is an important modulator of cytoskeleton organization during cell migration, for example. In migrating cells actin remodeling is tightly regulated and localized; therefore, how the
Sharmini Alagaratnam et al.
Molecular and cellular endocrinology, 306(1-2), 75-80 (2008-12-02)
Several genomic regions are recurrently over- or underrepresented in testicular germ cell tumours (TGCTs), but only a fraction of their genes change their expression accordingly. Two publications to date have studied DNA copy numbers and associated gene expression changes on
Toru Hirota et al.
Cell, 114(5), 585-598 (2003-09-19)
Aurora family kinases contribute to regulation of mitosis. Using RNA interference in synchronized HeLa cells, we now show that Aurora-A is required for mitotic entry. We found that initial activation of Aurora-A in late G2 phase of the cell cycle
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