SAB4200049

Anti-FRAT1 (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

• Synonym(s): Anti-Frequently rearranged in advanced T-cell lymphomas, Anti-GBP
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• mol wt: antigen ~29 kDa
• species reactivity: human, rat
• packaging: antibody small pack of 25 μL
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.5 mg/mL
• technique(s): western blot: 1.5-3 μg/mL using HEK-293T cell extract over expressing human FRAT1 and a rat skeletal muscle extract (S1 fraction)
• UniProt accession no.: Q92837
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FRAT1(10023)

Description

General description

Frequently rearranged in advanced T-cell lymphomas-1 (FRAT1) is a glycogen synthase kinase 3 β (GSK3β)-binding protein and belongs to the FRAT family. This protein contains a conserved GSK3β interacting domain. The FRAT1 gene is ubiquitously expressed and is mapped on the human chromosome at 10q24.1.

Specificity

Anti-FRAT1 (C-terminal) specifically recognizes human and rat FRAT1.

Application

Anti-FRAT1 (C-terminal) antibody produced in rabbit may be used in immunoblotting.

Biochem/physiol Actions

Frequently rearranged in advanced T-cell lymphomas-1 (FRAT1) protein plays a pivotal role in positively regulating the wingless-related integration site (Wnt)/β-catenin signaling pathway. This protein competes with axin for binding of glycogen synthase kinase 3 β (GSK3β), thus displacing GSK3β from the axin-β-catenin complex. The FRAT1 gene expression is upregulated in several human cancer lines primarily in gastric cancer. Overexpression of this gene is associated with esophageal squamous cell carcinoma (ESCC), ovarian, breast and cervical carcinoma. The FRAT1 gene expression is also observed in astrocytomas and human gliomas.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable