SAB4200021

Anti-OS9 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-ERLEC2, Anti-Endoplasmic reticulum associated protein, Anti-Osteosarcoma amplified 9
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~97/88 kDa
• species reactivity: rat, mouse, human
• packaging: antibody small pack of 25 μL
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using lysates of rat NRK cells; western blot: 4-8 μg/mL using whole extracts of human HEK-293T cells
• UniProt accession no.: Q13438
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... OS9(10956) ; mouse ... Os9(216440) ; rat ... Os9(362891)

Description

General description

OS9 endoplasmic reticulum lectin is encoded by the gene mapped to human chromosome 12q13.3-q14.1. Alternative splicing of the OS-9 gene yields two proteins, namely OS-9.1 and OS-9.2. These proteins are ubiquitously expressed in human tissues.

Specificity

Anti-OS9 recognizes human, rat, and mouse OS9 isoforms 1 and 2.

Application

Anti-OS9 antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

OS9 endoplasmic reticulum lectin is an endoplasmic reticulum (ER)-resident lectin involved in ER quality control. OS9 is upregulated in response to ER stress through activation of the Ire1/Xbp1 pathway. OS9 associates both with components of the ERAD machinery and ERAD substrates. OS9 binds to misfolding proteins and prevents their secretion from the ER. It then directs them to the suppressor/enhancer of Lin-12-like (Sel1L) / hydroxymethylglutaryl reductase degradation protein 1 (Hrd1) dislocation and ubiquitylation complex in the ER membrane. Two OS9 spliced variants, OS-9.1 and OS-9.2 expressions are amplified in tumors.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable