SAB4200012

Anti-TIM4 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-T-cell immunoglobulin and mucin domain-containing protein 4, Anti-T-cell membrane protein 4, Anti-TIMD4
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~45 kDa; antigen ~85 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): western blot: 1-2 μg/mL using whole extracts of HEK-293T cells overexpressing human TIM4
• UniProt accession no.: Q96H15
• shipped in: dry ice
• Storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TIMD4(91937)

Description

General description

T cell immunoglobulin and mucin domains containing protein 4 (TIM4, TIMD4) belong to the TIM family of proteins that are type I cell surface glycoproteins. It consists of an immunoglobulin variable (IgV) domain with six cysteines, a heavily glycosylated mucin-like domain, a transmembrane domain and a cytoplasmic domain. All TIMs contain a tyrosine phosphorylation motif in the cytoplasmic tail except for TIM4. It is expressed primarily on mature antigen-presenting cells (APCs) and is the natural ligand for TIM1.

The TIM4 gene is mapped to human chromosome 5q33.3. The encoded protein is exclusively expressed in antigen-presenting cells.

Specificity

Anti-TIM4 (N-terminal) recognizes human TIM4.

Application

Anti-TIM4 (N-terminal) antibody produced in rabbit may be used in immunoblotting.

Biochem/physiol Actions

T cell immunoglobulin and mucin domains containing protein 4 (TIM4, TIMD4) is a phosphatidylserine receptor that mediates phagocytosis of apoptotic cells. It is involved in the regulation of T helper cells (Th1 and Th2) immune responses. TIM4 binds apoptotic cells by recognizing phosphatidylserine through its immunoglobulin domain. TIM1/TIM4 interaction regulates T cell activation and proliferation.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable