SAB4200004

Anti-Exportin 5 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-Exp5, Anti-Ran-binding protein 21, Anti-XPO5
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~135 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed HEK-293T cells; western blot: 1-2 μg/mL using A431 cell lysates
• UniProt accession no.: Q9HAV4
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... XPO5(57510) ; mouse ... Xpo5(72322) ; rat ... Xpo5(363194)

Description

General description

Exportin 5, also known as XPO5, Exp5 or Ran-binding protein 21, is encoded by the gene mapped to human chromosome 6p21.1. XPO5 belongs to the human karyopherin (Kap)β family. Structurally, Exp-5 has a ring-shaped closed conformation.

Specificity

Anti-Exportin 5 (N-terminal), recognizes human Exportin 5.

Application

Anti-Exportin 5 (N-terminal) antibody produced in rabbit is suitable for use in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions

Exportin 5 exports RNA such as viral hairpin RNAs, tRNAs and pre-microRNAs, which are likely to be its major endogenous cellular RNA cargos. Loss-of-function mutation in the gene leads to microsatellite instability.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable