POU class 1 homeobox 1 (POU1F1/ Pit1), a POU-homeodomain transcription factor is mapped on human chromosome 3p11.2. It belongs to the POU family of transcription factors.
Specificity
Anti-POU1F1/PIT1 (C-terminal) recognizes human POU1F1/PIT1.
Application
Anti-POU1F1/PIT1 (C-terminal) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.
Biochem/physiol Actions
POU class 1 homeobox 1 (POU1F1/PIT1) plays a key role in pituitary development and hormone expression in mammals. It participates in the differentiation and proliferation of the anterior pituitary somatotrophs, lactotrophs, and thyrotropes cell lineages. Therefore, abnormalities of the Pit-1 gene results in the syndrome of combined pituitary hormone deficiency (CPHD), a disease characterized by the lack of prolactin, growth hormone, and thyroid stimulating hormone. POU1F1/PIT1 regulates its target gene expression by binding to response elements on their promoter regions and recruitment of co-regulatory proteins that alter histone acetylation and modify chromatin structures.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
American journal of medical genetics. Part A, 155A(9), 2242-2246 (2011-08-05)
Microdeletion syndromes include numerous syndromic phenotypes associated with intellectual disability and dysmorphic features. We report on a patient with a novel microdeletion of chromosomal region 3p11.2-p12.1 containing POU1F1, chromatin-modifying protein 2B (CHMP2B), and vestigial-like 3 (VGLL3) genes. Our patient was
The Journal of clinical endocrinology and metabolism, 86(4), 1545-1550 (2001-04-12)
The POU homeodomain containing transcriptional activator POU1F1, formerly called Pit1 or GHF-1, is required for the embryological determination and postnatal secretory function of the GH-, PRL-, and TSH-producing cells in the anterior pituitary. Several mutations in the gene encoding POU1F1
Proceedings of the National Academy of Sciences of the United States of America, 105(7), 2481-2486 (2008-02-15)
Enhancers have been functionally described for >35 years, but the molecular principles underlying the integration of regulatory inputs to alternate gene enhancers used during mammalian organogenesis remain incompletely understood. Using a combination of in vivo enhancer mapping and proteomics approaches
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