SAB3700943

Anti-Rabbit IgG (H+L)-Biotin antibody produced in chicken

affinity isolated antibody, lyophilized powder

• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: chicken
• conjugate: biotin conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: rabbit
• technique(s): immunohistochemistry: suitable; indirect ELISA: suitable; western blot: suitable
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

IgG (immunoglobulin G) consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50kDa and 25kDa respectively. The chains are interlinked with a disulfide bond. Both the L and the H chains consist of a variable domain at the N-terminal. The C-terminal bears′ constant domains: three in H chain with a hinge region and one in the L chain. IgG antibody is enriched in mother′s milk. IgG contributes to 10−20% of plasma protein and is regarded as one of the most abundant serum protein.

Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Biotin, Anti-Chicken Serum, Rabbit IgG and Rabbit Serum.

Immunogen

Rabbit IgG whole molecule

Application

Anti-Rabbit IgG (H+L)-Biotin antibody produced in chicken has been used in immunohistochemistry.

Biochem/physiol Actions

IgG (immunoglobulin G) antibody offers protection against bacterial, fungal and viral infections. IgG antibody share similar function with IgM antibody in complement system activation. It helps in opsonization, complement fixation and antibody-dependent cell-mediated cytotoxicity. IgG mediates hypersensitivity type II and type III. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Physical properties

Antibody format: IgG

Physical form

Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution

Reconstitute with 1.0 mL deionized water (or equivalent).

Safety Information

• pictograms: GHS06,GHS09
• signalword: Danger
• hcodes: H302,H311,H411
• pcodes: P280 - P301 + P312 + P330 - P302 + P352 + P312
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2
• supp_hazards: EUH032
• Storage Class: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable