SAB1300503

Monoclonal Anti-mobC antibody produced in mouse

purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-Mobc, Anti-Mobilization protein C
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 19CT.2.8.14, monoclonal
• form: buffered aqueous solution
• species reactivity: Neisseria gonorrhoeae
• technique(s): indirect ELISA: 1:1000; western blot: 1:100-1:500
• isotype: IgG1
• NCBI accession no.: AAO45531
• UniProt accession no.: Q84AT5
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

The mobilization region of the 7.4 kb beta-lactamase plasmid pSJ7.4 from Neisseria gonorrhoeae was characterized. The 3.2 kb HindIII-BamHI fragment of pSJ7.4 was mobilized between Escherichia coli strains by conjugative plasmid RK2. Selected restriction enzyme-generated deletions of this fragment were subcloned in pACYC177 to obtain constructs that were suitable for analysis of the mobilization region. Mating experiments showed that a highly conserved 1.9 kb DNA region within coordinates 4096-5997 is required for mobilization of pSJ7.4. This region contains two genes encoding the mobilization protein MobA, another protein, MobC, putatively involved in mobilization from some hosts, and an intergenic oriT. The 168 bp intergenic sequence also contains the promoters for mobA and mobC in an arrangement that suggests divergent transcription and autoregulation from oriT. The 56 kDa MobA was expressed in E. coli as a (6x)His-Tag fusion protein. Purified MobA specifically induced plasmid relaxation by nicking at the oriT. MobA is exceptional because the N-terminal region alone can mobilize pSJ7.4, albeit at a lower frequency than the full-length protein, even in the absence of MobC. The carboxyl terminal region of MobA did not share homology with other mobilization proteins, but may be involved in promoting efficient transfer of pSJ7.4.

Immunogen

MOBC (AAO45531, )
This antibody was raised using purified His-tagged recombinant full length Neisseria gonorrhoeae plasmid pSJ7.4 mobilization protein C (mobC)and mobilization protein A (mobA).

Physical form

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable