SAB1300301

Anti-SULT1B1 (N-term) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-1B, Anti-Cytosolic, Anti-Member 1, Anti-Sulfotransferase 1B2, Anti-Sulfotransferase family
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: human, mouse
• technique(s): indirect ELISA: 1:1000; western blot: 1:100-1:500
• NCBI accession no.: NP_055280
• UniProt accession no.: O43704
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SULT1B1(27284)

Description

General description

Cytosolic sulfotransferase (ST) enzymes catalyze the sulfate conjugation of many drugs, xenobiotic compounds, hormones, and neurotransmitters. Sulfotransferases such as SULT1B1 catalyze the biotransformation of a large number of endogenous compounds such as neurotransmitters, steroids, bile acids, and thyroid hormones, as well as drugs and xenobiotics. SULT1B1 contains a binding site for the sulfate donor, 3-prime-phosphoadenosine 5-prime-phosphosulfate, and a cysteine residue conserved in the ST1 gene family of sulfotransferases. SULT1B1 shares between 51.5% and 56.3% amino acid sequence identity with members of the phenol sulfotransferase gene family. Northern blot analysis detects 4 SULT1B1 transcripts between 79 kb and 13 kb expressed at highest levels in liver, peripheral blood leukocytes, colon, small intestine, and spleen. Lower expression is detected in lung, placenta, and thymus, and no expression is detected in other tissues. Recombinant rat and human SULT1B1, expressed in E. coli, catalyze sulfation of p-nitrophenol, 3,3-prime,5-triiodothyronine (T3), and dopamine, but not of beta-estradiol and dehydroepiandrosterone. SULT1B1 shows higher affinities for formation of T3 sulfate than do the phenol sulfotransferases ST1A3 (SULT1A1) or ST1A5. Bacterially-expressed SULT1B1 sulfates small phenols such as 1-naphthol and p-nitrophenol and thyroid hormones, including 3,3-prime-diiodothyronine, triiodothyronine, reverse triiodothyronine, and thyroxine. No activity is detected against several sterols or dopamine.

Immunogen

SULT1B1 (NP_055280, 1-30)
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SULT1B1.

Physical form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable