SAB1300006

Anti-PRDM16 (N-term) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-PFM13, MEL1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: human
• technique(s): immunohistochemistry: 1:50-1:100; indirect ELISA: 1:1000
• NCBI accession no.: NP_071397
• UniProt accession no.: Q9HAZ2
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PRDM16(63976)

Description

General description

PR/SET domain 16 (PRDM16) is encoded by the gene mapped to human chromosome 1p36.32. PRDM16 is a 140kDa zinc-finger protein characterized with a PR (PRD1-BF1-RIZ1 homologous)-domain. It is highly expressed in brown fat cells compared to white fat cells.

The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36,q21)-positive MDS/AML. The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal PR domain. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Alternatively spliced transcript variants encoding distinct isoforms have been reported. Purified recombinant GST fusion protein encoding N-terminal aa 13-316 of human PRDM16.

Immunogen

PRDM16 (NP_071397, 1-350)
Purified recombinant GST fusion protein encoding N-terminal aa 13-316 of human PRDM16.

Application

Anti-PRDM16 (N-term) antibody produced in rabbit has been used in western blotting..

Biochem/physiol Actions

PR/SET domain 16 (PRDM16) is a potential transcriptional regulator. It regulates a bidirectional cell fate switch between skeletal myoblasts and brown fat cells. The encoded protein plays an essential role in promoting adipogenesis by co-activating transcriptional function of PPARγ. PRDM16 is required for cardiac development. Thus, loss of PRDM16 gene expression leads to the development of cardiomyopathy of 1p36 deletion syndrome. Variation in the gene expression is associated with the development of migraine.

Physical form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable