S9450

Anti-Sam68 (N-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-Gap-associated tyrosine phosphoprotein, 62-KD, Anti-KH domain-containing, RNA-binding, signal transduction-associated protein 1, Anti-KHDR1, Anti-KHDRBS1, Anti-Src-associated in mitosis 68 kDa protein, Anti-p68
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• form: buffered aqueous solution
• mol wt: antigen ~68 kDa
• technique(s): western blot: 1:500-1:1000 using using K562 lys
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... KHDRBS1(10657)

Description

General description

Src-associated in mitosis 68 kDa protein (Sam68/KHDR1) or K homology (KH) domain-containing, RNA binding, signal transduction associated 1 (KHDRBS1) is a member of the signal transducer and activator of RNA (STAR) family of RNA-binding proteins. This protein contains a KH (hnRNP K homology) RNA-binding domain located within a larger domain of 200 amino acids with RNA binding activity named GSG (GRP33/Sam68/GLD-1) domain. The Sam68 gene is mapped to human chromosome 1p35.

Specificity

Anti-Sam68 (N-terminal) recognizes human Sam68.

Application

Anti-Sam68 (N-terminal) antibody produced in rabbit may be used in immunoblotting.

Biochem/physiol Actions

Src-associated in mitosis 68 kDa protein (Sam68/KHDR1/KHDRBS1) participates in signal transduction, RNA metabolism, apoptosis, transcription, cell cycle, and regulation. Besides binding to RNA and its modulator proteins, Sam68 contains proline-rich motifs and multiple potential tyrosine phosphorylation sites that are involved in the binding to proteins with Src homology 3 (SH3) and SH2 domains. It controls the activation of nuclear transcription factor kappa B (NF-κB).

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.