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Product S9059 was manufactured using passive conjugation of the STP with 10 nm gold colloid. It does not involve covalent or linker chemistry. The protein binds to the gold colloid through amino acid residues, including charge attraction (lysine), hydrophobic attraction (tryptophan), and sulfur bonding (cysteine).
S9059
Streptavidin−Gold from Streptomyces avidinii
~10 nm nominal (colloidal gold), buffered aqueous glycerol solution
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0.25 ML
$204.00
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$262.00
$204.00
Available to ship onApril 29, 2025Details
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0.25 ML
$204.00
1 ML
$262.00
About This Item
$204.00
Available to ship onApril 29, 2025Details
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conjugate
gold conjugate
Quality Level
form
buffered aqueous glycerol solution
concentration
~2.5 A520 units/mL
nominal
~10 nm (colloidal gold)
mean particle size
8-12 nm (monodisperse)
storage temp.
2-8°C
target post-translational modification
unmodified
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General description
Strepavidin-collodial gold conjugate binds to molecules, such as primary antibodies, that have been previously conjugated with biotin.
Streptavidin is a protein produced by the bacterium Streptomyces avidinii. It has four binding sites for biotin as does avidin. Both have been used extensively as probes in immunochemical systems, conjugated to antibodies, enzymes, fluorochromes and gold. Avidin is highly basic (pI ~ 10.5), compared to streptavidin (pI ~ 5-6). Avidin has a slightly higher binding capacity for biotin than streptavidin does, but it has glycosylated side chains which give higher backgrounds in blotting techniques than does streptavidin, which is not a glycoprotein. Streptavidin is more resistant than avidin to dissociation into subunits by guanidinium chloride. Streptavidin may be conjugated to colloidal gold.
Application
For detection of biotinylated compounds.
Strepavidin-collodial gold conjugate is a component of streptavidin/colloidal gold-biotin detection systems use in immunoblotting, light microscopy, and immunoelectron microscopy applications. It is commonly used in postembedding immunocytochemistry.
Physical form
Suspension in 0.01 M phosphate buffered saline, pH 7.2, containing 1% BSA, 20% glycerol, and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
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John S Mitchell et al.
Analytical biochemistry, 343(1), 125-135 (2005-06-14)
Surface plasmon resonance (SPR) biosensor formats using gold nanoparticle or protein signal amplification for the sensitive assay of small molecules were developed using progesterone as a model compound. Progesterone was immobilized to a dextran surface in the Biacore biosensor through
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Can you provide details on how streptavidin is conjugated to gold nanoparticles? Specifically, I'd like to know if there is any covalent binding involved or if it's a passive binding process.
1 answer-
Helpful?
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I want to know the streptavidin concentration in this stock. (e.g. 0.5 mg/mL)
1 answer-
This information is reported as the protein concentration in the lot-specific Certificate of Analysis. A review of lots available in the last 7 years reveal a range of 11 - 11.6 ug/mL. Please see the link below to review a sample or lot-specific Certificate of Analysis:
https://www.sigmaaldrich.com/US/en/product/sigma/s9059#product-documentationHelpful?
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