S8950

Anti-Synemin antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-DMN, Anti-Desmuslin, Anti-SYN, Anti-SYNM
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~180 kDa (also a band at ~80 kDa)
• species reactivity: rat
• concentration: ~1.5 mg/mL
• technique(s): western blot: 1.5-3 μg/mL using rat brain extract (S1 fraction)
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SYNM(23336) ; mouse ... Synm(233335) ; rat ... Synm(308709)

Description

General description

Synemin (SYNM), also known as desmuslin/DMN is a unique type VI intermediate filament (IF) protein. The synemin gene codes for three isoforms, such as a-synemin, b-synemin and L-synemin with overlapping distribution. The three isoforms share the same head and rod domains but differ at their C-terminus. These isoforms are expressed in muscle and brain, in astrocytes, glia and neuronal cells and in the vascular system. In human skeletal myofibers, b-synemin is located to the high stress regions. SYNM gene is located on human chromosome 15q26.3.

Specificity

Anti-Synemin specifically recognizes rat synemin.

Application

Anti-Synemin antibody produced in rabbit may be used in immunoblotting.

Biochem/physiol Actions

Synemin protein interacts with major intermediate filament (IF) proteins including desmin, vimentin and glial fibrillary acidic protein (GFAP) and is thought to play an important role in maintaining structural integrity of the cell. In astrocytoma with migratory properties, synemin contributes to the actin cytoskeleton dynamics. This protein play a major role as a bridging protein between IFs and focal adhesions in rat and human liver tissues. Synemin may also participate in cytoskeletal cross?linking. This IF?associated protein is also known to play a key role in human muscle diseases.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.