S8945

Anti-Syntaxin 8 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• MDL number: MFCD02264138
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 27 kDa
• species reactivity: rat
• technique(s): immunoprecipitation (IP): 10-20 μg using NRK cell lysate; microarray: suitable; western blot: 2-4 μg/mL using whole cell extract of the rat kidney NRK cell line and the rat pheochromocytoma PC12 cell line
• UniProt accession no.: Q9Z2Q7
• shipped in: dry ice
• Storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... STX8(9482) ; mouse ... Stx8(55943) ; rat ... Stx8(59074)

Description

General description

Syntaxin 8 (Stx8) is a member of the soluble NSF (N-ethyl-maleimide-sensitive protein) attachment proteins receptor (t-SNARE) family. Syntaxin 8 shares 23% identity with syntaxin 6 and is ubiquitously expressed in multiple tissues, with highest levels in the heart. This protein is anchored to membranes by a C-terminal hydrophobic domain and appears to contain a helical domain. Stx8 is localized to membrane compartments of the early endosome (EE). This protein has two unique di-leucine-based motifs. Stx8 gene is mapped to human chromosome 17p12.

Specificity

Anti-Syntaxin 8 specifically recognizes rat syntaxin 8 (27 kDa).

Immunogen

synthetic peptide corresponding to amino acids 117-134 located at the mid-region of rat syntaxin 8, conjugated to KLH. This sequence is identical in mouse syntaxin 8 and highly conserved (two amino acid substitutions) in human syntaxin 8.

Application

Anti-Syntaxin 8 antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Syntaxin 8 (Stx8) protein has been suggested to function in clathrin-independent vesicular transport and membrane fusion events necessary for protein transport from the early endosome (EE) to the late endosome (LE). This protein may also participate in vesicular trafficking and docking.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.