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S8629

Sigma-Aldrich

Sulfatase from Patella vulgata (keyhole limpet)

Type V, essentially salt-free, lyophilized powder, ≥5 units/mg solid

Synonym(s):

Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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About This Item

CAS Number:
9016-17-5
Enzyme Commission number:
3.1.6.1 (BRENDA, IUBMB)
EC Number:
232-772-1
MDL number:
MFCD00132399
UNSPSC Code:
12352204
NACRES:
NA.54

type

Type V

form

essentially salt-free, lyophilized powder

specific activity

≥5 units/mg solid

foreign activity

β-Glucuronidase ≤2 unit/mg solid

storage temp.

−20°C

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Application

Sulfatase from Patella vulgata has been used in a study to validate a method for the quantitation of quercetin from human plasma. Sulfatase from Patella vulgata has also been used in a study to compare properties of human lung SRS-A to distinct fractions from ionophore-induced rat mononuclear cell SRS.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C (30 min assay).

substrate

Product No.
Description
Pricing

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Slow reacting substances: comparison of some properties of human lung SRS-A and two distinct fractions from ionophore-induced rat mononuclear cell SRS.
M K Bach et al.
Journal of immunology (Baltimore, Md. : 1950), 122(1), 160-165 (1979-01-01)
D J Smith et al.
Journal of animal science, 80(5), 1240-1249 (2002-05-22)
Ractopamine HCl is a beta-adrenergic leanness-enhancing agent recently approved for use in swine. Depletion of ractopamine in tissues, and elimination of ractopamine and its metabolites in urine, is of interest for the detection of off-label use. The objectives of this
I Erlund et al.
Journal of chromatography. B, Biomedical sciences and applications, 727(1-2), 179-189 (1999-06-09)
A validated method for the quantitation of trace levels of quercetin from human plasma to be used in pharmacokinetic and biomarker studies is presented. Quercetin conjugates were hydrolysed enzymatically, plasma proteins were removed using a Bond Elut C18 extraction column
Bradley J Stevenson et al.
Drug testing and analysis, 7(10), 903-911 (2015-03-06)
The hydrolysis of sulfate ester conjugates is frequently required prior to analysis for a range of analytical techniques including gas chromatography-mass spectrometry (GC-MS). Sulfate hydrolysis may be achieved with commercial crude arylsulfatase enzyme preparations such as that derived from Helix
Beat B Fischer et al.
BMC plant biology, 10, 279-279 (2010-12-21)
When photosynthetic organisms are exposed to harsh environmental conditions such as high light intensities or cold stress, the production of reactive oxygen species like singlet oxygen is stimulated in the chloroplast. In Chlamydomonas reinhardtii singlet oxygen was shown to act
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