S8571

Anti-Seladin-1 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-24-dehydro-cholesterol reductase (DCHR24), Diminuto/Dwarf-1 homolog, Anti-Selective Alzheimer’s Disease Indicator-1
• MDL number: MFCD09265376
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~60 kDa
• species reactivity: human
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): western blot: 1:4,000-1:8,000 using HEK293 cells expressing human seladin-1.
• UniProt accession no.: Q15392
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... DHCR24(1718)

Description

General description

Seladin-1 (selective Alzheimer′s disease indicator-1) is an anti-apoptotic gene. It is a human homolog of the Diminuto/Dwarf1 gene described in plants andC. elegans. Seladin-1 is encoded by the DHCR24 (3-b-hydroxysterol-D-24-reductase) gene, a flavin-adenine-dinucleotide (FAD)-dependent oxidoreductase. It is mainly located in the endoplasmic reticulum.

Immunogen

synthetic peptide corresponding to amino acids 68-85 of human seladin-1, conjugated to KLH.

Application

Anti-Seladin-1 antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Seladin-1 (selective Alzheimer′s disease indicator-1) catalyzes the conversion of desmosterol to cholesterol. It prevents apoptosis via inhibition of caspase-3, a key mediator of the apoptotic cascade, suggesting that seladin-1 may be involved in the regulation of cell survival and death. Decreased expression of seladin-1 in specific neurons is linked to Alzheimer′s disease (AD). Seladin-1 functions as a key mediator of Ras-induced senescence in mouse and human fibroblasts. It protects neurons from β-amyloid toxicity and oxidative stress. Seladin-1 is involved in cholesterol homeostasis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)