Human Sin3A is a 1273 amino acids protein containing paired amphipathic helix (PAH) domains, important for protein-protein interactions.
SIN3A (switch-insensitive 3 family member A) is a transcriptional repressor and MeCP2 (methyl-CpG binding protein 2) interactor. It is located on human chromosome 15q24. The product encoded by SIN3A gene is a constituent of the histone deacetylase (HDAC) complex.
Immunogen
synthetic peptide corresponding to amino acids 1267-1282 of human Sin3A, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide is conserved in human, mouse, and rat and is not present in Sin3B.
Application
Anti-Sin3A, C-Terminal antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.
Biochem/physiol Actions
SIN3A (switch-insensitive 3 family member A) along Sin3B is essential for transcription and growth suppressor functions of the mitotic spindle assembly checkpoint protein (Mad1) and interferon-induced GTP-binding protein (Mx1). It also controls cellular functions like differentiation, oncogenesis, apoptosis and cell fate determination. The Sin3A/HDAC corepressor comprises Sin3A along with histone deacetylases (HDAC1 and 2), pRB-associated proteins (RbAp46, RbAp48) and Sin3A Associated Protein 30 h(SAP30). Repression of transcription by the Sin3A/HDAC complex interaction with O-GlcNAc transferase (OGT) leads to the inactivation of transcription factors and RNA Polymerase II.
SIN3A (switch-insensitive 3 family member A) plays an important role in the growth of early embryos. It helps in the multiplication and survival of various cells by suppressing diverse signaling pathways. It acts as a transcriptional regulator for the formation of cortical brain. Absence of Sin3A can suppress the development of breast cancer cell by elevating apoptosis.
Physical form
Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Haploinsufficiency of MeCP2-interacting transcriptional co-repressor SIN3A causes mild intellectual disability by affecting the development of cortical integrity
Witteveen JS, et al.
Nature Genetics, 48(8), 877-887 (2016)
Maximum growth and survival of estrogen receptor-alpha positive breast cancer cells requires the Sin3A transcriptional repressor
Ellison-Zelski SJ and Alarid ET
Molecular Cancer, 9 (2010)
Identification and characterization of three new components of the mSin3A corepressor complex
Fleischer TC, et al.
Molecular and Cellular Biology, 23(10), 3456-3467 (2003)
Decreased expression of the SIN3A gene, a candidate tumor suppressor located at the prevalent allelic loss region 15q23 in non-small cell lung cancer
Suzuki H, et al.
Lung Cancer, 59(1), 24-31 (2008)
Recruitment of O-GlcNAc transferase to promoters by corepressor mSin3A: coupling protein O-GlcNAcylation to transcriptional repression
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