S6071

Anti-Synphilin-1 (C-terminal) antibody produced in rabbit

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-SNCAIP, Anti-Synuclein-α-interacting protein
• MDL number: MFCD04040605
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 130 kDa
• species reactivity: human
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~2 mg/mL
• technique(s): western blot: 0.25-0.5 μg/mL using HEK-293 cells expressing human synphilin-1
• UniProt accession no.: Q9Y6H5
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... SNCAIP(9627) ; mouse ... Sncaip(67847) ; rat ... Sncaip(307309)

Description

General description

Synphilin-1 (synuclein a-interacting protein 1, SNCAIP), is a cytoplasmic protein with six ankyrin-like repeats, coiled-coil domain, and an ATP/GTP binding domain.[1] It is highly expressed in brain, heart, placenta[2] and presynaptic nerve terminals.[3] The synphilin gene produces at least nine transcript variants encoding seven distinct proteins.

Immunogen

synthetic peptide corresponding to amino acids 911-919 of human synphilin-1, conjugated to KLH. This sequence is identical in several species including mouse, rat, and dog synphilin-1.

Application

Anti-Synphilin-1 (C-terminal) antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Cells with this mutation are more prone to staurosporine-induced cell death highlighting its cytoprotective functionality.[3] Synphilin-1 associates with and is ubiquitinated by several proteins, including α -synuclein, parkin, dorfin and siah E3 ubiquitin protein ligase 1 (SIAH1),[4] and is a major component of Lewy bodies in Parkinson′s disease (PD).[3] In synaptic vesicles it is modulated by α-synuclein.[3] The central domain of synphilin-1 has been suggested to be required for the formation of aggregates and cytotoxicity.[5] Synphilin-1 association with α -synuclein promotes the formation of cytosolic inclusions.[6] In human postmortem brain tissue, synphilin-1, like a-synuclein is present in neurophil.[7] Mutation of synphilin-1 in PD patients at amino acid R621C, is implicated in the sequestration, ubiquitination and proteasomal inhibition leading to increased accumulation of toxic intermediates.[3]

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable