immunoprecipitation (IP): 4 μg using STAT1 from 0.5–1 mg of human Raji and Jurkat, and mouse spleen cell lysates western blot (chemiluminescent): 1 μg/mL using human Raji, Jurkat, and Hut, or mouse CTLL and spleen cell lysates
GST fusion protein containing the C-terminal of human STAT1, amino acids 714-750, expressed in Escherichia coli.
Physical form
Solution in 0.07 M Tris-glycine, pH 7.4, containing 0.105 M NaCl, 0.035% sodium azide, and 30% glycerol.
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Among the diverse co-regulatory relationships between transcription factors (TFs) and microRNAs (miRNAs), feedback loops have received the most extensive research attention. The co-regulation of TFs and miRNAs plays an important role in colorectal cancer (CRC) growth. Here, we show that
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