S4697

Monoclonal Anti-Seladin-1 antibody produced in mouse

~2 mg/mL, clone SL-14, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-24-dehydro-cholesterol reductase (DCHR24), Anti-Diminuto/Dwarf-1 homolog, Anti-Selective Alzheimer’s disease indicator-1
• MDL number: MFCD09265376
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: SL-14, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~60 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: suitable; indirect ELISA: suitable; western blot: 1-2 μg/mL using extracts of HEK-293T cells expressing human seladin-1
• isotype: IgG2b
• UniProt accession no.: Q15392
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... DHCR24(1718)

Description

General description

Monoclonal Anti-Seladin-1 (mouse IgG2b isotype) is derived from the hybridoma SL-14 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to human seladin-1.Seladin-1 is a human homolog of the Diminuto/Dwarf1 gene described in plants and C elegans. Seladin-1 (selective alzheimer′s disease indicator-1, 60 kDa) is encoded by the DHCR24 (3-β-hydroxysterol-Δ-24- reductase) gene. Seladin-1 is mainly located in the endoplasmic reticulum.

Immunogen

synthetic peptide corresponding to amino acids 505-516 of human seladin-1, conjugated to KLH.

Application

Monoclonal Anti-Seladin-1 antibody produced in mouse has been used in:

• enzyme linked immunosorbent assay (ELISA),
• immunoblotting
• immunocytochemistry

Biochem/physiol Actions

Seladin-1 (selective Alzheimer′s disease indicator-1, 60 kDa), is an anti-apoptotic protein, found to be down regulated in large pyramidal neurons in brain regions affected by Alzheimer′s disease (AD). It is a flavin-adenine-dinucleotide (FAD)-dependent oxidoreductase, involved in cholesterol homeostasis. It catalyzes the conversion of desmosterol to cholesterol. It prevents apoptosis via inhibition of caspase-3, a key mediator of the apoptotic cascade, suggesting that seladin-1 may be involved in the regulation of cell survival and death. Seladin-1 has been shown to function as a key mediator of Ras-induced senescence in mouse and human fibroblasts. In response to oncogenic and oxidative stress, seladin-1 binds to p53 and displaces the E3 ubiquitin ligase Mdm2 from p53, resulting in accumulation of p53. The expression of seladin-1 has been reported to be up-regulated in adrenocortical adenomas and in some tumors.

Physical form

Solution in 0.01 M phophate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter