S4688

Anti-α1-Syntrophin (AG-17) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-SNTA1
• MDL number: MFCD03454687
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 60 kDa
• species reactivity: rat
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using 0.5% paraformaldehyde fixed/0.5% Triton X-100 treated rat skeletal muscle; microarray: suitable; western blot: 1:1,000 using rat skeletal muscle homogenate extract (membrane fraction)
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SNTA1(6640) ; rat ... Snta1(362242)

Description

General description

Syntrophins are expressed in a wide range of tissues. α1-Syntrophin (SNTA1) contain two pleckstrin (PH) homology domains, found in a wide array of signaling proteins. SNTA1 gene is located on human chromosome 20q11.2. In rat skeletal muscle, α1-syntrophins are mainly found to be located on the sarcolemma.

Specificity

Anti-a1-Syntrophin (AG-17) recognizes a1-syntrophin (60 kDa).

Immunogen

synthetic peptide located at the N-terminus of human α1-syntrophin (amino acids 2-18 with C-terminally added lysine) conjugated to KLH.

Application

Anti-α1-Syntrophin (AG-17) antibody produced in rabbit may be used in immunoblotting and immunohistochemistry.

Biochem/physiol Actions

Syntrophins associate with the dystrophin complex to act as modular adapters and helps in recruiting signaling proteins to the membrane. The pleckstrin (PH) homology domains of α1-Syntrophin (SNTA1) mainly helps in mediating this recruiting action. Mutation on this gene is accountable for long QT syndrome 12 (LQT12), an autosomal-dominant manner.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Storage and Stability

For continuous use, store at 2 °C - 8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable