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S4521

Sigma-Aldrich

Saponin from Quillaja sp.

Sapogenin content 20-35 %

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10 G
$65.10
25 G
$132.00

About This Item

CAS Number:
8047-15-2
EC Number:
232-462-6
MDL number:
MFCD00081981
UNSPSC Code:
12161902
NACRES:
NA.32

$65.10

Available to ship onMay 01, 2025Details

Request a Bulk Order

description

non-ionic

composition

Sapogenin content, 20-35%

technique(s)

HPLC: suitable
protein quantification: suitable

sulfated ash

≤10%

SMILES string

O1[C@H]([C@@H]([C@H]([C@@H]([C@H]1CO)O)O)O)O[C@H]2[C@@H](O[C@@H]([C@H]([C@@H]2O)O[C@@H]8O[C@@H]([C@H]([C@@H]([C@H]8O)O)O)CO)C(=O)O)O[C@@H]3[C@@](C4[C@@](C5[C@]([C@@]6(C[C@H]([C@]7([C@H]([C@@H](C(CC7C6=CC5)(C)C)OC(=O)\C(=C/C)\C)OC(=O)C)CO)O)C)(CC4)C)(CC3)C

InChI

1S/C55H86O24/c1-10-23(2)46(71)79-43-44(72-24(3)60)55(22-59)26(17-50(43,4)5)25-11-12-30-51(6)15-14-32(52(7,21-58)29(51)13-16-53(30,8)54(25,9)18-31(55)61)75-49-41(77-48-38(67)36(65)34(63)28(20-57)74-48)39(68)40(42(78-49)45(69)70)76-47-37(66)35(64)33(62)27(1

InChI key

AXNVHPCVMSNXNP-ZELRDNAQSA-N

General description

Saponins are steroid or triterpenoid glycosides. They are biosurfactants that have several uses in the food and cosmetic industry.[1]

Application

Saponin from Quillaja bark has been used:
  • to stain fertilized oocytes[2]
  • in the analysis of intracellular cytokine production[3]
  • to permeabilize dendritic cells[4]

Biochem/physiol Actions

Potent hemolytic when injected i.v.; surfactant that enhances penetration of proteins and other macromolecules through cell membranes; it also has been used as an adjuvant for vaccines.
Saponins solubilize the low density lipoprotein (LDL) fraction of cholesterol in micelles, thus lowering their concentration. Saponin from Quillaja bark is used as a flavoring agent in food and beverages. This saponin has been shown to enhance immune-cell proliferation in vitro.[1][5]

Preparation Note

Purified to remove low molecular weight contaminants 

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319,H335

Hazard Classifications

Eye Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000443623
0000443623
0000441022
0000441021
0000441021

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Adjuvant effects of saponins on animal immune responses.
Rajput ZI
Journal of Zhejiang University. Science. B, 8(3) , 153-161 (2007)
Louis Lignereux et al.
Preventive veterinary medicine, 157, 70-77 (2018-08-09)
Contagious caprine pleuropneumonia (CCPP), caused by Mycoplasma capricolum subsp. capripneumoniae, has long been considered a goat-specific disease. Since 2007 there has been growing evidence that this disease can affect wild ungulates either kept in captivity or in the wild. In
R Martin-Ibañez et al.
Human reproduction (Oxford, England), 23(12), 2744-2754 (2008-08-22)
Human embryonic stem cells (hESCs) have potential use in clinical therapy and regenerative medicine. One of the major challenges regarding the application of these cells is the development of an efficient cryopreservation protocol, since current methods, which include slow-freezing-rapid thawing
Yvonne Lange et al.
Biochemistry, 48(36), 8505-8515 (2009-08-07)
A few membrane-intercalating amphipaths have been observed to stimulate the interaction of cholesterol with cholesterol oxidase, saponin and cyclodextrin, presumably by displacing cholesterol laterally from its phospholipid complexes. We now report that this effect, referred to as cholesterol activation, occurs
Subset-dependent modulation of dendritic cell activity by circovirus type 2.
Vincent IE
Immunology, 115(3), 388-398 (2005)
View All Related Papers

Questions

1–3 of 3 Questions  

  1. Is this product suitable for downstream molecular tests if used in a lysis buffer for DNA extraction ?

    1 answer

    1. This product has not been tested for suitability with any molecular tests after being used in a DNA lysis buffer. Validation by the intended user is necessary, as this has not been assessed. Researchers associated with Radboud University Medical Center have reported the usage of product 47036, or a molecular biology grade saponin, for bacterial DNA isolation prior to qPCR. For more details, refer to the citation below.

      Optimized DNA isolation method for microbiome analysis of human tissues
      Carlijn Bruggeling, Daniel R. Garza, Soumia Achouiti, Wouter Mes, Bas E. Dutilh, Annemarie Boleij
      bioRxiv 2020.08.25.267641; doi: https://doi.org/10.1101/2020.08.25.267641

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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