S4191

Monoclonal Anti-SLIPR/MAGI-3 antibody produced in mouse

clone SLP-32, purified immunoglobulin, buffered aqueous solution

• MDL number: MFCD03792433
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: SLP-32, monoclonal
• form: buffered aqueous solution
• species reactivity: rat
• technique(s): immunocytochemistry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1-2 μg/mL using rat brain extract
• isotype: IgG1
• UniProt accession no.: Q9JK71
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: rat ... Magi3(245903)

Description

General description

Monoclonal Anti- SLIPR/MAGI-3 (mouse IgG1 isotype) is derived from the SLP-32 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice. Membrane-associated guanylate kinase, WW and PDZ domain-containing 3 (MAGI-3) protein belong to the membrane-associated guanylate kinase (MAGUK) family. This protein is made up of a guanylate-kinase-like region, six post-synaptic density-95/discs large/zonula occludens-1 (PDZ), and two WW domains. MAGUKs localizes to regions of cell-cell contact, such as tight junctions in epithelial cells and synaptic junctions in neurons. The MAGI3 gene is associated with the human chromosome at 1p13.2.

Specificity

Monoclonal Anti-SLIPR/MAGI-3 recognizes rat SLIPR/MAGI-3 (approx. 160 kDa).

Immunogen

C-terminal SLIPR protein fused to GST (amino acids 421-785) of rat SLIPR.

Application

Monoclonal Anti-SLIPR/MAGI-3 antibody produced in mouse may be used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry

Biochem/physiol Actions

Membrane-associated guanylate kinase, WW and PDZ domain-containing 3 (MAGI-3) protein serve to position the phosphatase to specific subcellular locations that are involved in the regulation of cell proliferation and survival. This protein interacts with the protein phosphatase and tensin homolog (PTEN) and receptor protein tyrosine phosphatase β (RPTPβ). MAGUKs are involved in the assembly of multiprotein complexes via their protein-protein interaction domains.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.