S3573

Anti-SPHK2 (696-710) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-sphingosine kinase 2
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~69 kDa
• species reactivity: human
• technique(s): western blot: 1:500-1:2,000
• UniProt accession no.: Q9NRA0
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SPHK2(56848)

Description

General description

SPHK (sphingosine kinase) has two isoforms, namely SPHK1 and SPHK2 (sphingosine kinase 1 and 2). SPHK2 is abundantly expressed in liver, kidney, brain and heart. It is located on chromosome 19q13.

Immunogen

synthetic peptide corresponding to amino acids 696-710 of human SPHK2.

Application

Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit
sigma.com/htcb-if.

Biochem/physiol Actions

SPHK2 (sphingosine kinase 2) induces the invasion and multiplication of colorectal cancer cell by increasing the expression of MYC (protooncogene). Reactions of all-trans-retinoic acid (ATRA) on colon cancer cells enhances due to improper expression of SphK2.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)