S2687
Anti-SDF-1β/PBSF antibody produced in goat
affinity isolated antibody, lyophilized powder
Synonym(s):
Anti-Stromal Cell-Derived Factor-1β/Pre-B Cell Growth Stimulating Factor
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About This Item
MDL number:
UNSPSC Code:
51111800
biological source
goat
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
human
technique(s)
indirect ELISA: 0.5-1.0 μg/mL
neutralization: suitable
western blot: 0.1-0.2 μg/mL
UniProt accession no.
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CXCL12(6387)
General description
Stromal cell-derived factor-1 (SDF-1) is a proinflammatory cytokine that belongs to the CXC family of chemokines. SDF-1 exists as two isoforms, SDF-1β and SDF-1α. The effects of SDF-1 are mediated by its binding to the receptor CXCR4, and sometimes to CXCR7. Both lymphoid (bone marrow, spleen and thymus) and non-lymphoid (stomach, kidney, liver, lung, brain, heart) organs express SDF-1. The most important functions assigned to SDF-1 are angiogenesis, migration of μglia and astrocytes, tissue repair and as chemoattractant to hematopoietic cells. Studies also implicate SDF-1 in survival of human glioma cells via the activation of PI3K/Akt pathway. The other pathways that are activated by SDF-1 include MAPK, NF-κB, paxillin, Crk and other focal adhesion proteins. A variety of human malignancies of pancreas, breast, prostate and glioma exhibit overexpression of CXCR4. High expression of SDF-1 has been observed in lymph nodes, lungs, bone and liver that are the most common sites of metastasis of breast cancer cells. Anti- SDF-1β/PBSF recognizes recombinant human SDF-1β/PBSF. By immunoblotting, this antibody exhibits less than 5% cross-reactivity with recombinant human SDF-1α.
Immunogen
recombinant human SDF-1β/PBSF expressed in E. coli.
Application
Anti-SDF-1β/PBSF antibody may be used for immunoblotting at a working concentration of 0.1-0.2 μg/ml. For ELISA, the recommended working antibody concentration is 0.5-1.0 μg/ml. The antibody is also suitable for neutralization assays; ND50 of the antibody is ~5-15 μg/ml.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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R K Ganju et al.
The Journal of biological chemistry, 273(36), 23169-23175 (1998-08-29)
The alpha-chemokine stromal cell-derived factor (SDF)-1alpha binds to the seven transmembrane G-protein-coupled CXCR-4 receptor and acts to modulate cell migration and proliferation. The signaling pathways that mediate the effects of SDF-1alpha are not well characterized. We studied events following SDF-1alpha
T Nagasawa et al.
Seminars in immunology, 10(3), 179-185 (1998-07-07)
PBSF/SDF-1 is a CXC chemokine which has unique functions among chemokines. It is essential for viability of the embryo, B lymphopoiesis, bone marrow hematopoiesis and cardiogenesis. A receptor for PBSF/SDF-1 was shown to be CXCR4 that is an entry co-receptor
D Tseng et al.
British journal of cancer, 104(12), 1805-1809 (2011-05-19)
Local recurrence of glioblastomas is a major cause of patient mortality after definitive treatment. This review discusses the roles of the chemokine stromal cell-derived factor-1 and its receptor CXC chemokine receptor 4 (CXCR4) in affecting the sensitivity of glioblastomas to
Yan Zhou et al.
The Journal of biological chemistry, 277(51), 49481-49487 (2002-10-22)
Chemokines were described originally in the context of providing migrational cues for leukocytes. They are now known to have broader activities, including those that favor tumor growth. We addressed whether and which chemokines may be important promoters of the growth
A Müller et al.
Nature, 410(6824), 50-56 (2001-03-10)
Breast cancer is characterized by a distinct metastatic pattern involving the regional lymph nodes, bone marrow, lung and liver. Tumour cell migration and metastasis share many similarities with leukocyte trafficking, which is critically regulated by chemokines and their receptors. Here
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