S2319

Anti-SNAP-23 (DS-19) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• MDL number: MFCD05665256
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 23 kDa
• species reactivity: mouse
• technique(s): indirect immunofluorescence: 1:200 using mouse embryonic 3T3-L1 cell line; western blot: 1:1,000 using whole cell extract of mouse fibroblasts NIH3T3 cell line
• UniProt accession no.: O09044
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SNAP23(8773) ; mouse ... Snap23(20619) ; rat ... Snap23(64630)

Description

General description

Synaptosomal-associated protein, 23 kDa, syndet (SNAP23), belonging to the SNAP25 family genes is a non-neuronal isoform of synaptosomal-associated protein 25 (SNAP-25), originally identified in a human B-lymphocyte cDNA library in a yeast two-hybrid screen for proteins interacting with syntaxin 4. SNAP-23 is ubiquitously expressed and is localized mainly to the plasma membrane. The primary structure of SNAP-23 is 59% identical to SNAP-25. It contains a central cluster of cysteine residues and predicted coiled-coil segments. It is a tSNARE which has two tandem soluble N-ethylmale-imide-sensitive factor-attachment protein receptor (SNARE) motifs. SNAP23 gene has eight exons and is located on human chromosome 15q21−22.

Specificity

Anti-SNAP-23 (DS-19) recognizes SNAP-23 (23 kDa).

Immunogen

synthetic peptide corresponding to amino acids 2-20 of mouse SNAP-23, conjugated to KLH.This sequence is highly conserved in rat (90% identity) and human (78% identity) SNAP-23.

Application

Anti-SNAP-23 (DS-19) antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Synaptosomal-associated protein, 23 kDa, syndet (SNAP23) plays a vital role in various protein trafficking events in non-neuronal cells. It is mainly involved in polarized protein trafficking, glucose transporter type 4 (GLUT4) trafficking in adipocytes, compound exocytosis in mast cells and platelet dense core granule release. This gene acts as an oncogene in ovarian cancer (OC). This gene is capable of producing two forms of message, such as the full length message (SNAP23A) and a shorter message (SNAP23B).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable