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S2313

Supelco

Streptavidin−Fluorescent Polymer, Ultrasensitive

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About This Item

UNSPSC Code:
12352200

storage temp.

−20°C

General description

Streptavidin (SA) and a chelate antennae moiety are covalently conjugated to a polymer backbone. Multiple Terbium ions chelated to each polymer chain amplify the time-resolved fluorescence signal.

Application

Detection of biotinylated proteins and nucleic acids and other biomolecules. Suitable for use in multiwell plate and other solid surface based assays including ELISA and Western blotting.

Features and Benefits

  • Time resolved fluorescent signal allows improved signal to noise ratio
  • Demonstrates higher sensitivity than other fluorescent labels
  • Low level of photo-bleaching
  • Dual incorporation of lanthanide chelate and antennae molecule provides direct fluorescent output

Quantity

250 μg is enough for 2-5 plates or blots.

Physical form

Supplied as 100× concentrate in 50% glycerol, buffer pH 7.4.

Other Notes

Sensitivity: Detects a minimum of 1 ng biotinylated compound in ELISA format.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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E F Dickson et al.
Journal of photochemistry and photobiology. B, Biology, 27(1), 3-19 (1995-01-01)
The principles and practice of the application of time-resolved lanthanide chelate luminescence (or fluorescence) as a detection method for ultrasensitive bioanalytical assays such as immunoassays and nucleic acid hybridization assays are reviewed. The various lanthanide chelate-based detection systems which have

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