S1700

Anti-Synip (N-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-STX4-interacting protein, Anti-STXBP4, Anti-Syntaxin 4-interacting protein, Anti-Syntaxin-binding protein 4
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~65 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.5 mg/mL
• technique(s): western blot: 0.2-0.4 μg/mL using HEK-293T cell lysate expressing human Synip and 0.3-0.6 mg/mL using a HepG2 cell lysate
• UniProt accession no.: Q6ZWJ1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... STXBP4(252983)

Description

General description

Syntaxin-4 interacting protein (STXBP4) is also known as synip. It is expressed at high level in insulin-secreting pancreatic β cell line, βHC-9 cells. STXBP4 gene is located on human chromosome 17q22.

Specificity

Anti-Synip (N-terminal) specifically recognizes human Synip.

Application

Anti-Synip (N-terminal) antibody produced in rabbit may be used in immunoblotting.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Biochem/physiol Actions

Syntaxin-4 interacting protein (STXBP4) serve as functional links between the insulin signaling cascade and insulin-responsive glucose transporter (GLUT4) vesicles. It interacts with syntaxin 4. Synip inhibits the translocation of GLUT4 from intracellular vesicles to the plasma membrane by binding to syntaxin 4 and preventing the interaction between syntaxin 4 and vesicle-associated membrane protein (VAMP2). STXBP4 can control ΔNp63 ubiquitination. Hence it may act as a squamous cell carcinoma (SCC) biomarker.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)