S1694
Stemline® T Cell Expansion Medium
Serum-free, Without L-glutamine, liquid, sterile-filtered, suitable for cell culture
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.81
Recommended Products
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
shipped in
wet ice
storage temp.
2-8°C
General description
Developed to promote the optimal expansion of adult human T cells,[1] Stemline T Cell Expansion Medium demonstrates significantly greater expansion (55%) when compared to alternative media, and viability greater than 95%. Additionally, flow cytometry confirms that with Stemline, a proper CD4/CD8 ratio is maintained. In an ex vivo functional assay (51Chromium Release Assay), T cells expanded in Stemline medium proved to be highly functional and possessed cytolytic potential greater than T cells expanded in serum-containing medium (RPMI with 10% fetal bovine serum). In an in vivo functional assay (GvHD Induction), human T lymphocytes expaned in Stemline medium were injected into NOD/SCIDb2M mice (n=12). Engraftment, perivascular infiltration, and lethal GvHD were observed by day 15 in 100% of mice, demonstrating excellent in vivo expansion and functionality.
Stemline T Cell Expansion Medium is free of serum and all other animal-derived components with the exceptions of human serum albumin and transferrin. This exclusion increases performance consistency and elimates safety risks associated with potential adventitious agents.
Produced in a GMP state-of-the-art facility, Stemline T-Cell Expansion Medium is clearly an excellent choice for your T-cell applications.
Stemline T Cell Expansion Medium is free of serum and all other animal-derived components with the exceptions of human serum albumin and transferrin. This exclusion increases performance consistency and elimates safety risks associated with potential adventitious agents.
Produced in a GMP state-of-the-art facility, Stemline T-Cell Expansion Medium is clearly an excellent choice for your T-cell applications.
Application
Stemline® T Cell Expansion Medium has been used to generate silver (Ag)-specific T-cells.[1]
Components
The medium does not contain antibiotics or cytokines. Human serum albumin and transferrin are the only human origin material and are non-reactive (donor level) for anti-HIV 1 & 2, anti-HCV and HBsAg.
Reconstitution
Add 20 mL of 200 mM L-glutamine solution or 0.584 g of γ-irradiated L-glutamine powder per liter of solution.
Legal Information
Stemline is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Beatriz M Carreno et al.
Science (New York, N.Y.), 348(6236), 803-808 (2015-04-04)
T cell immunity directed against tumor-encoded amino acid substitutions occurs in some melanoma patients. This implicates missense mutations as a source of patient-specific neoantigens. However, a systematic evaluation of these putative neoantigens as targets of antitumor immunity is lacking. Moreover
Amino-Terminal Extended Peptide Single-Chain Trimers Are Potent Synthetic Agonists for Memory Human CD8+ T Cells
Beatriz M. Carreno
Journal of Immunology, 188(12), 5839-5849 (2012)
Bruno Nervi et al.
Experimental hematology, 35(12), 1823-1838 (2007-09-04)
Graft-vs-host disease (GVHD) is the major cause of morbidity and mortality after allogeneic hematopoietic stem cell transplantation. Models of immunodeficient mice that consistently and efficiently reconstitute with xenoreactive human T cells would be a valuable tool for the in vivo
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