S1686
Monoclonal Anti-v-Src antibody produced in mouse
clone 327, purified immunoglobulin, lyophilized powder
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About This Item
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
327, monoclonal
form
lyophilized powder
mol wt
antigen ~56 kDa
species reactivity
human, rat, mouse
technique(s)
immunoprecipitation (IP): suitable (suitable; may appear as doublet due to phosphorylation)
western blot: 2-5 μg/mL using COLO201 cell extract
isotype
IgG1κ
UniProt accession no.
shipped in
wet ice
Storage temp.
2-8°C
Gene Information
human ... SRC(6714)
mouse ... Src(20779)
rat ... Src(83805)
General description
Src family kinases are a group of structurally related non-receptor protein tyrosine kinases that can lead to a variety of cellular responses including differentiation and proliferation. All the members have an amino terminal that has the kinase function and the C-terminal that regulates the kinase function. The amino terminal has Src homology 4 domain (SH4), containing a myristylation signal sequence essential for membrane localization; conserved SH3 and SH2 domains, which mediate protein-protein interactions, separated from the SH4 by a unique region; a catalytic kinase domain. The major difference between v-Src and c-Src is the structure of the C terminal. Src is involved in the regulation of many cellular functions such as migration, adhesion, gene transcription, cell cycle, apoptosis and differentiation. Few signalling pathways through which Src regulates these processes are PI3K, STAT3, FAK and paxillin. Src overexpression is observed in human cancers.
Monoclonal Anti-v-Src reacts with active Src kinase from human, mouse, and rat detecting a band of approximately 56 kDa.
Monoclonal Anti-v-Src reacts with active Src kinase from human, mouse, and rat detecting a band of approximately 56 kDa.
Immunogen
Src protein (p60src).
Application
The recommended anti-v-Src antibody concentration is 2-5 μg/mL for immunoblotting using a COLO201 cell extract and chemiluminescence detection. The antibody is suitable for immunoprecipitation.
Physical form
Lyophilized with 100 μg bovine serum albumin.
Analysis Note
p60src may appear as a doublet due to phosphorylation.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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R B Irby et al.
Oncogene, 19(49), 5636-5642 (2000-12-15)
Since the original identification of a transmissible agent responsible for the development of tumors in chickens, now known to be a retrovirus encoding the v-src gene, significant progress has been made in defining the potential functions of its human homolog
Valerie G Brunton et al.
Cancer research, 65(4), 1335-1342 (2005-03-01)
Src tyrosine kinase expression and activity are elevated during colon cancer progression. How this contributes to the malignant phenotype is not fully understood. We show that in KM12C colon carcinoma cells, expression of kinase-deficient Src proteins (SrcMF and Src251) does
P T Ram et al.
Oncogene, 20(13), 1601-1606 (2001-04-21)
Extracellular signals when routed through signaling pathways that use heterotrimeric G proteins can engage multiple signaling pathways leading to diverse biological consequences. One locus at which signal sorting occurs is at the level of G proteins. G protein alpha-subunits appear
P L Schwartzberg
Oncogene, 17(11 Reviews), 1463-1468 (1998-10-21)
c-src was first isolated as the normal cellular homologue of v-src, the transforming gene of Rous Sarcoma virus (Stehelin et al., 1976). As the first proto-oncogene described and one of the first molecules demonstrated to have tyrosine kinase activity, Src
S M Thomas et al.
Annual review of cell and developmental biology, 13, 513-609 (1997-01-01)
Src family protein tyrosine kinases are activated following engagement of many different classes of cellular receptors and participate in signaling pathways that control a diverse spectrum of receptor-induced biological activities. While several of these kinases have evolved to play distinct
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