S1576

Anti-SDCBP (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-MDA-9, Anti-Melanoma differentiation associated protein-9, Anti-Pro-TGF-α−cytoplasmic domain-interacting protein 18, Anti-ST1, Anti-SYCL, Anti-Syndecan binding protein (syntenin), Anti-Syntenin, Anti-TACIP18
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~30 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using lysates of human HeLa cells; indirect immunofluorescence: 10-20 μg/mL using human HeLa cells; western blot: 2-4 μg/mL using whole extracts of human HEK-293T cells
• UniProt accession no.: O00560
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SDCBP(6386)

Description

General description

Syndecan binding protein (SDCBP) is also named as syntenin and melanoma differentiation associated gene-9 (MDA-9) contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. SDCBP is located to membrane associated adherens junctions and focal adhesions. It is present in the endoplasmic reticulum and nucleus. SDCBP gene is mapped to human chromosome 8q12.1 and has 9 exons and is expressed in fetal kidney, liver, lung and brain.

Specificity

Anti-SDCBP (N-terminal) recognizes human SDCBP.

Application

Anti-SDCBP (N-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions

Syndecan binding protein (SDCBP) might distress the organization of cytoskeletal-membrane and the stimulation of transcription factors. It may also affect cell adhesion and protein trafficking. SDCBP promotes metastasis in human melanoma cells by activating c-Src. Alternative splicing results in multiple transcript variants encoding different isoforms. Syntenin enables the recruitment of ubiquitinated proteins to its transmembrane partners by interacting with ubiquitin through is C- and N-terminal regions. It plays a key role in synapses formation and maturation. Upregulation of syntenin blocks human immunodeficiency virus-1 (HIV-1) production and HIV-mediated cell fusion. Depletion of this gene enhances the entry of HIV-1.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.