S0696

Anti-Striatin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• MDL number: MFCD02264125
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: 110 kDa
• species reactivity: mouse, rat
• technique(s): microarray: suitable; western blot: 1.5-3.0 μg/mL using whole cell extract of mouse fibroblast NIH3T3 cell line; western blot: 3-5 μg/mL using mouse brain and rat brain extract (S1 fraction)
• UniProt accession no.: P70483
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... STRN(6801) ; mouse ... Strn(268980) ; rat ... Strn(29149)

Description

General description

Striatin (STRN) is also termed as calmodulin-binding protein. Heart, kidney, several digestive organs and the adrenal shows its expression. STRN is located on human chromosome 2p22.2. It is a neuronal, membrane associated protein. Striatin is multimodular and belongs to a family of Ca²⁺ and calmodulin binding proteins with WD motif repeats, that includes SG2NA and zinedin. Striatin is found in low abundance in the rat brain. It is mostly expressed in neurons of the motor and olfactory structures of the central nervous system, predominantly in the striatum. It is found to be expressed in the somatodendritic compartment of neurons and in dendritic spines.

Specificity

Anti-Striatin recognizes mouse and rat striatin, 110 kDa.

Immunogen

synthetic peptide corresponding to amino acids 378-396 located in the mid region of mouse striatin, conjugated to KLH. This sequence is highly conserved in rat and human striatin (84-89% identity).

Application

Anti-Striatin antibody produced in rabbit may be used in immunoblotting.

Biochem/physiol Actions

Striatin (STRN) forms a complex with protein phosphatase 2A (PP2A) and has been shown to function as an estrogen receptor alpha (ERα)-binding protein. It plays a key role as a molecular anchor which helps to localize ERα to the membrane. STRN also aid in systematizing the ERα-endothelial NOS (eNOS) membrane signaling complex. It has been suggested that striatin is an adaptor or transducer protein involved in Ca²⁺ and calmodulin-dependent signaling events within dendritic spines. Down regulation of striatin, which is expressed in a few subsets of neurons, impairs the growth of dendrites as well as locomotor activity.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.