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Key Documents

S0638

Sigma-Aldrich

Serum Replacement 1 (50×)

liquid, sterile-filtered, suitable for cell culture

Synonym(s):

50× Serum Replacement, Cell Culture Serum Substitute, Replacement Serum

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71
sterility:
sterile-filtered
form:
liquid
Pricing and availability is not currently available.

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

Application

Serum Replacement 1 (50×) has been used as a supplement in Ham′s/F-12 medium for tumour cells.[1] It has also been used as a supplement to culture Jurkat T cells in RPMI medium.(2)

Other Notes

Defined, multi-purpose serum replacement useful in studying the effects of growth factors and the production of proteins. Contains highly purified, heat-treated bovine serum albumin, heat-treated bovine transferrin and bovine insulin. Does not contain growth factors, steroid hormones, glucocorticoids, cell adhesion factors, detectable Ig and mitogens. May be used with anchorage-dependent and suspension cells.

Caution

Stable for 30 days when diluted in cell culture medium.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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Proliferation and intracellular glutathione in Jurkat T cells with concentrated whey protein products
Middleton N, et al.
International Journal of Dairy Technology, 13(7), 565-573 (2003)
Constanze M Hammerle et al.
PLoS genetics, 16(10), e1009069-e1009069 (2020-10-16)
The genetic mechanisms that determine the size of the adult pancreas are poorly understood. Imprinted genes, which are expressed in a parent-of-origin-specific manner, are known to have important roles in development, growth and metabolism. However, our knowledge regarding their roles

Questions

  1. What is the difference between Serum Replacement 1, 2, and 3?

    1 answer
    1. These concentrated serum replacements are defined, as multipurpose products designed to eliminate the need for serum in culture. These products do not contain growth factors, steroid hormones, or mitogens.

      S0638 Serum Replacement 1, 50X
      Total protein concentration at 1X of 770 mg/mL
      Highly purified bovine serum albumin (heat treated)
      Bovine transferrin (heat treated)
      Bovine insulin

      S9388 Serum Replacement 2, 50X
      Total protein concentration at 1X of 570 mg/mL
      Highly purified bovine serum albumin (heat treated)
      Bovine transferrin (heat treated)
      Bovine insulin

      S2640 Serum Replacement 3, 50X
      Total protein concentration at 1X of 664 mg/mL
      Highly purified human serum albumin (heat treated)
      Human transferrin (heat treated)
      Human (recombinant) insulin

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