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RAB1052

Sigma-Aldrich

Human GHRL/Ghrelin ELISA Kit

for cell culture supernatants, plasma, and serum samples

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96 WELLS
$674.00

$674.00


Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)


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96 WELLS
$674.00

About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

$674.00


Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type plasma
sample type serum sample(s)
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 8 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... GHRL(51738)

General description

This ELISA antibody pair detects Human Ghrelin (GHRL)

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached Protocolfor details.

Biochem/physiol Actions

The peptide ghrelin, one of the cleavage products of prepropeptide produced from the GHRL (growth hormone-releasing peptide) gene, functions in stimulating food intake and controlling energy balance. It has potent stimulating effects on the secretion of growth hormone. GHRL serves as an endogenous ligand for the growth hormone secretagogue receptor 1α (GHSR 1α). The protein regulates body weight on long-term and appetite on short-term. Preadipocyte differentiation stimulated by ghrelin is found to increase BMI and negatively regulate the anorexigenic effect of leptin. Polymorphism in the GHRL gene is linked to variations in blood pressure, high-density lipoproteins, low-density lipoproteins, serum cholesterol, blood glucose and metabolic syndrome.[1][2]

Other Notes

A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number.

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable


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Shafqat R Chaudhry et al.
Brain stimulation, 12(3), 643-651 (2019-02-13)
To assay peripheral inter-ictal cytokine serum levels and possible relations with non-invasive vagus nerve stimulation (nVNS) responsiveness in migraineurs. This double-blinded, sham-controlled study enrolled 48 subjects and measured headache severity, frequency [headache days/month, number of total and mild/moderate/severe classified attacks/month]
T F Lobo et al.
Journal of endocrinological investigation, 42(2), 149-156 (2018-04-27)
The study of adipokines in overweight women with early-onset (diagnosed before 20 weeks) gestational diabetes mellitus (GDM) could help to understand the ethiopathological mechanisms of this disorder. Our aim was to assess adipokine levels in overweight pregnant women with early-onset GDM
Beate Leppert et al.
Nature communications, 11(1), 561-561 (2020-02-13)
Parabens are preservatives widely used in consumer products including cosmetics and food. Whether low-dose paraben exposure may cause adverse health effects has been discussed controversially in recent years. Here we investigate the effect of prenatal paraben exposure on childhood overweight
A Lindqvist et al.
Molecular and cellular endocrinology, 511, 110835-110835 (2020-05-07)
It is not known how ghrelin affects insulin secretion in human islets from patients with type 2 diabetes (T2D) or whether islet ghrelin expression or circulating ghrelin levels are altered in T2D. Here we sought out to identify the effect
Kalina Duszka et al.
PloS one, 15(4), e0232099-e0232099 (2020-04-25)
Food cues affect hunger and nutritional choices. Omnipresent stimulation with palatable food contributes to the epidemics of obesity. The objective of the study was to investigate the impact of food cues on appetite-related hormones and to assess the functionality of

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