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RAB0012

Sigma-Aldrich

Phospho-Akt (pSer473) / pan-Akt ELISA Kit

for detection of human, mouse or rat phospho-akt (pSer473) and pan-akt in cell and tissue lysates

Synonym(s):

Akt ELISA Kit

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human, mouse, rat

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type: human cell lysate
sample type tissue lysate

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... AKT1(207)

General description

The Phospho-Akt (pSer473) ELISA (Enzyme-Linked Immunosorbent Assay) kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates.

Immunogen

Akt (pSer473) synthetic phosphopeptide, Recombinant Human EGFR

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

pictograms

Corrosion
GHS05

signalword

Warning

hcodes

H290

Hazard Classifications

Met. Corr. 1

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Fatima Maqoud et al.
International journal of molecular sciences, 19(8) (2018-08-22)
The effects of Ca2+-activated K⁺ (BK) channel modulation by Paxilline (PAX) (10-7⁻10-4 M), Iberiotoxin (IbTX) (0.1⁻1 × 10-6 M) and Resveratrol (RESV) (1⁻2 × 10-4 M) on cell cycle and proliferation, AKT1pSer473 phosphorylation, cell diameter, and BK currents were investigated
Molly Stanley et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 36(46), 11704-11715 (2016-11-18)
Hyperinsulinemia is a risk factor for late-onset Alzheimer's disease (AD). In vitro experiments describe potential connections between insulin, insulin signaling, and amyloid-β (Aβ), but in vivo experiments are needed to validate these relationships under physiological conditions. First, we performed hyperinsulinemic-euglycemic

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