R9779

Anti-RhoB antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• MDL number: MFCD07370477
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~21 kDa
• species reactivity: mouse, human, rat
• concentration: ~1.5 mg/mL
• technique(s): western blot: 1-2 μg/mL using HEK293 cells expressing human RhoB.
• UniProt accession no.: P62745
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... RHOB(388)

Description

General description

Rho GTPases belong to the Ras superfamily of small GTPases, which consists of the Rho, small GTP binding protein (Rac) and cell division cycle 42 (Cdc42) subgroups. Ras homolog family member B (RhoB) is mapped to human chromosome 2p24.1. It is present in the endosomal compartment. The farnesylated form present in multivesicular late endosomes and geranylgeranylated form localizes in plasma membrane. The C-terminal hypervariable domain contains the CAAX box prenylation motif, a polybasic Lys-rich domain, and may contain additional Cys residues required for palmitoylation.

Immunogen

synthetic peptide corresponding to amino acids 177-188 located near the C-terminus of human RhoB.

Application

Anti-RhoB antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Ras Homolog Family Member B (RhoB) modulates Rac1 trafficking and may play a key role in maintaining the integrity of endothelial cells during inflammation. It functions as tumor suppressor and its absence is implicated in tumors like cervical, breast cancer and clear cell renal cell carcinoma (ccRCC). RhoB favors neural crest formation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)