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R9529

Sigma-Aldrich

Anti-AtRAC3 antibody produced in rabbit

~1.2 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Arabidopsis ROP (Rho of plants)

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 21 kDa

species reactivity

Arabidopsis sp.

concentration

~1.2 mg/mL

technique(s)

western blot: 0.25-0.5 μg/mL using recombinant AtRac3 expressed in E. coli

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Arabidopsis thaliana ... Rop1(824293)

Specificity

Recognizes Arabidopsis AtRAC3 expressed in E. coli.

Immunogen

synthetic peptide corresponding to amino acids 61-79 located in a region near the N-terminus of Arabidopsis AtRAC3, conjugated to KLH. This sequence is identical in AtRACs 1, 2, 4, 5, 6, 8, 10, and 11 and also in RAC/Rop of many plant species. It is highly conserved (single amino acid substitution) in AtRAC7 and AtRAC9.

Physical form

Solution in 0.01 M phosphjate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store ammonium sulfate suspension at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Reconstituted and diluted antiserum should be stored in aliquots at −20 °C.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificates of Analysis (COA)

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Nadav Sorek et al.
Molecular and cellular biology, 37(23) (2017-09-13)
ROPs or RACs are plant Rho-related GTPases implicated in the regulation of a multitude of signaling pathways that function at the plasma membrane via posttranslational lipid modifications. The relationships between ROP activation status and membrane localization has not been established.

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