R9404
Anti-RhoA antibody produced in rabbit
~2 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-p21 Rho
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About This Item
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~21 kDa
species reactivity
monkey, mouse, human
concentration
~2 mg/mL
technique(s)
western blot: 1-2 μg/mL using HEK293 cells expressing human RhoA/ Hela cell extract
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RHOA(387)
General description
RhoA (ras homolog family member A) belongs to the Rho GTPase family of proteins. It is located on chromosome 3p21.31.
Specificity
Anti-RhoA recognizes human RhoA.
Immunogen
synthetic peptide corresponding to amino acids 177-189 located near the C-terminus of human RhoA.
Biochem/physiol Actions
In multiciliated epithelial cells, RhoA (ras homolog family member A) controls the actin network dynamics. It also modulates membrane protrusion and contractility in the cell body. It is one of the essential factor for Ras transformation. It is associated with cancer metastasis.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in "frost-free" freezer, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. For continuous use, store at 2-8°C for up to one month.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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GPX1 (Glutathione Peroxidase 1)
Kulak M V and ,Weigel R J
Atlas of Genetics and Cytogenetics in Oncology and Haematology, 18(7) (2014)
Activation of Rac1, RhoA, and mitogen-activated protein kinases is required for Ras transformation
Khosravi-Far R, et al.
Molecular and Cellular Biology, 15(11), 6443-6453 (1995)
Chia-Hsin Chan et al.
Nature cell biology, 12(5), 457-467 (2010-04-13)
The RhoA GTPase is crucial in numerous biological functions and is linked to cancer metastasis. However, the understanding of the molecular mechanism responsible for RhoA transcription is still very limited. Here we show that RhoA transcription is orchestrated by the
Bruno Souza Bonifácio et al.
The Journal of eukaryotic microbiology, 69(3), e12903-e12903 (2022-03-14)
Cellular invasion by Trypanosoma cruzi metacyclic trypomastigotes (MTs) or tissue culture trypomastigotes (TCTs) is a complex process involving host-parasite cellular and molecular interactions. Particularly, the involvement of host cell actin cytoskeleton during trypomastigote invasion is poorly investigated, and still, the
RhoA regulates actin network dynamics during apical surface emergence in multiciliated epithelial cells
Sedzinski J, et al.
Journal of Cell Science, 130(2), 420-428 (2017)
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