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R9001

Sigma-Aldrich

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)

Type X, lyophilized powder

Synonym(s):

Transfer RNA, tRNA

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
41106305
NACRES:
NA.55

grade

for molecular biology

type

Type X

form

lyophilized powder

storage temp.

−20°C

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General description

Transfer RNA (tRNA) is isolated from baker′s yeast by phenol-chloroform extraction and ethanol precipitation.

Application

Suitable for use as a carrier in nucleic acid purification and precipitation.

Components

tRNA is provided as a lyophilized powder.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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Christophe Maris et al.
Nucleic acids research, 48(8), 4521-4537 (2020-03-15)
The polypyrimidine tract binding protein (PTB) is a multi-domain protein involved in alternative splicing, mRNA localization, stabilization, polyadenylation and translation initiation from internal ribosome entry sites (IRES). In this latter process, PTB promotes viral translation by interacting extensively with complex
A simplified method for combined immunohistochemistry and in-situ hybridization in fresh-frozen, cryocut mouse brain sections.
Newton, S.S., et al.
Brain Res. Protoc., 9, 214-214 (2002)
Mammalian in vitro splicing assays.
A Mayeda et al.
Methods in molecular biology (Clifton, N.J.), 118, 315-321 (1999-11-05)
Rahul Sharma et al.
STAR protocols, 1(1), 100006-100006 (2020-10-29)
DNA-FISH remains the method of choice to visualize genomic regions in situ ranging from a single locus to entire chromosomes. Current methods to generate probes rely on expensive kits that vary in labeling efficiency and are limited by the size
Fu-Jung Lin et al.
PLoS genetics, 9(5), e1003516-e1003516 (2013-05-30)
Mouse Ikbkap gene encodes IKAP--one of the core subunits of Elongator--and is thought to be involved in transcription. However, the biological function of IKAP, particularly within the context of an animal model, remains poorly characterized. We used a loss-of-function approach

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