R7404

Anti-RAD9 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-hRAD9
• MDL number: MFCD02263102
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 50 kDa (also band at 60 kDa)
• species reactivity: human
• technique(s): western blot: 1:500-1:1,000 using extracts of 293T cells transfected with RAD9
• UniProt accession no.: Q99638
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RAD9A(5883)

Description

General description

RAD9 checkpoint clamp component A (RAD9A), an important member of the 911 complex is mapped to human chromosome 11q13.

Specificity

Anti-RAD9 specifically recognizes recombinant RAD9 (50 and 60 kDa).

Immunogen

synthetic peptide corresponding to amino acid residues 376-391 of human RAD9, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide corresponds to RAD9A and is not present in RAD9B.

Application

Anti-RAD9 antibody produced in rabbit may be used in western blotting.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

RAD9 checkpoint clamp component A (RAD9A) participates in human cellular responses to DNA damage. RAD9 is found to possess 3′ to 5′ exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. RAD9 can be phosphorylated by ataxia-telangiectasia mutated (ATM) in response to DNA damage. It helps to stimulate S-phase checkpoint by modulating the phosphorylation of cell cycle checkpoint protein (RAD17), checkpoint kinases (Chks 1 and 2) Chk1, Chk2 and structure maintenance of chromosome 1 (Smc1) in response to replication block and DNA damage. It helps to maintain the stability of genome. In addition to its DNA repair role, it also plays a major role in radio resistance, androgen receptor signaling repression and immunoglobulin class switching. RAD9 also participates in telomere maintenance.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)